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yabA [2017-10-10 09:06:41]
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yabA [2017-10-10 09:06:41]

inhibitor of DnaA oligomerization, tethers DnaA to the polymerase-clamp protein DnaN
locus
BSU00330
pI
6.07
mw
13.96 kDa
protein length
119 aa Sequence Blast
gene length
357 bp Sequence Blast
function
positioning of DnaA
product
negative regulator of replication initiation
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • Gene

    Coordinates
    42,499 → 42,858

    Phenotypes of a mutant

  • strongly reduced growth rate in minimal medium [Pubmed|12060778]

    • The protein

    Catalyzed reaction/ biological activity

  • negative control of replication initiation [Pubmed|12060778]
  • associates with the ''oriC'' region in a [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]-dependent manner, this limits the amount of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] that can bind the ''oriC'' region [Pubmed|21895792]
  • YabA inhibits co-operative DNA binding by [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|21895792]
  • inhibits oligomerization and helix formation of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|23909787]

    • Protein family

  • yabA family (according to Swiss-Prot)

    • [SW|Domains]

  • leucine zipper

    • [SW|Cofactors]

  • Zinc [Pubmed|26615189]

    • Effectors of protein activity

  • [protein|83E05071DEC874248D3E96B8F3A093C65939B314|DnaN] overexpression or release from the replisome decreases association of [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|YabA] with ''oriC'', increases association of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] with ''oriC'' [Pubmed|21895792]

    • Structure

  • [PDB|5DOL] [Pubmed|26615189]

    • [SW|Localization]

  • forms foci in the midcell region, this depends on the interaction with [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] and [protein|83E05071DEC874248D3E96B8F3A093C65939B314|DnaN] [Pubmed|26615189]
  • co-localizes with the origin region and replication forks throughout the cell cycle [pubmed|28166228]

    • Expression and Regulation

    Operons

    genes
    [gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]-[gene|5231A0EE9C5A7556817B686D5B2B7B72B675BCAC|yaaN]-[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]-[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
    description
    [Pubmed|22383849]

    regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase [Pubmed|23490197]
    • view in new tab

    genes
    [gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B900 (yabA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1899 NBRP B. subtilis, Japan]
  • BKE00330 (Δ[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTATCCAAGGTTCCACACC, downstream forward: _UP4_TAGGGAAAAGGCTTCCGCAT
  • BKK00330 (Δ[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTATCCAAGGTTCCACACC, downstream forward: _UP4_TAGGGAAAAGGCTTCCGCAT

    • Labs working on this gene/protein

  • [SW|Philippe Noirot], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 Homepage]
  • [SW|Alan Grossman], Cambridge, MA, USA [http://web.mit.edu/biology/www/facultyareas/facresearch/grossman.html Homepage]

    • References

    Reviews

  • 20157337,28075389

    • Original publications

  • 19578359,21895792,12060778,16461910,19737352,19081080,23909787,22383849,26615189,28166228